PIRA PCR designer for restriction analysis of single nucleotide polymorphisms

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PIRA PCR designer for restriction analysis of single nucleotide polymorphisms

UNLABELLED Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, ...

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Single Nucleotide Polymorphisms and Association Studies: A Few Critical Points

Uncovering DNA sequence variations that correlate with phenotypic changes, e.g., diseases, is the aim of sequence variation studies. Common types sequence variations are Single nucleotide polymorphism (SNP, pronounced snip).SNPs are the third-generation molecular marker. SNP represents a DNA sequence variant of a single base pair with the minor allele occurring in more than 1% of a given popula...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2001

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/17.9.838